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Determination of Starch in White Card Paper by Double Enzymolysis-DNS Colorimetric Method
Received:September 06, 2024  
DOI：10.11980/j.issn.0254-508X.2025.03.016
Key Words:white card paper  starch  α-amylase  glucoamylase  determination
Fund Project:国家自然科学基金（32071722）。
Author NameAffiliationPostcode
LIU Wenting China Tobacco Hubei Industry Co.， Ltd.， Wuhan， Hubei Province， 430040 430040
ZHANG Shenghua China Tobacco Hubei Industry Co.， Ltd.， Wuhan， Hubei Province， 430040 430040
LI Chenqiao China Tobacco Hubei Industry Co.， Ltd.， Wuhan， Hubei Province， 430040 430040
SUN Peng Wuhan Hongjinlong Printing Co.， Ltd.， Wuhan， Hubei Province， 430100 430100
WANG Peng* Hubei Key Lab of Green Light Industrial Materials， Hubei University of Technology， Wuhan， Hubei Province， 430068 430068
AN Junjian Hubei Key Lab of Green Light Industrial Materials， Hubei University of Technology， Wuhan， Hubei Province， 430068 430068
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Abstract:To determine the starch content in white card paper， Glucoamylase and α-amylase were used to degrade the starch synergistically. Glucose of the enzyme hydrolysis product in the solution was measured by 3，5-dinitrosalicylic acid colorimetry （DNS） colorimetry and the starch content in white card paper was calculated. Based on the single factor experiment， response surface method was carried out to investigate the effects of key parameters， and the quadratic term model was established. The results of regression analysis presented a non-significant lack of fit （P<0.000 1） and a high coefficient of determination value （R2=0.988 6） for the response surface model. The optimum determination conditions of starch using double enzymolysis-DNS colorimetry were double enzyme addition amount of 5 mg/mL， enzymatic time of 75 min， enzymatic temperature of 40 ℃. The relative standard deviations and standard recovery of the method were 0.8% and 96.2%~98.0%， respectively.
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