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Study on Separation, Purification and Antioxidant Activities of Hemicellulose Polysaccharides from Eucalyptus Pre-hydrolysate |
Received:November 05, 2021 |
DOI:10.11980/j.issn.0254-508X.2022.04.005 |
Key Words:pre-hydrolysate polysaccharides separation and purification antioxidant activity |
Fund Project:国家自然科学基金青年科学基金项目(31700516);天津市制浆造纸重点实验室开放基金(202005)。 |
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Abstract:The hemicellulose polysaccharides (PHLP) were extracted from the eucalyptus wood pre-hydrolysate (PHL) by alcohol precipitation, and were separated and purified by DEAE-650M ion exchange column. Meanwhile, the two purified components neutral polysaccharide PHLP-1 and acidic polysaccharide PHLP- 2 were analyzed. The results of monosaccharide components showed that both PHLP-1 and PHLP-2 were composed of arabinose, xylose, galactose, mannose and glucose, with a molar ratio of 0.09∶1∶0.13∶0.07∶0.35 and 0.1∶1∶0.03∶0.09∶0.08. The infrared spectroscopy showed that both components had characteristic absorption peaks of polysaccharides. The scanning electron microscopy analysis showed that the structures of PHLP-1 and PHLP-2 were disordered and messy. The results of antioxidant experiments showed that the two purified polysaccharides had certain antioxidant properties and there was a dose-effect relationship. The order of antioxidant ability was: PHLP-2>PHLP-1>PHLP. |
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