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Study on Separation, Purification and Antioxidant Activities of Hemicellulose Polysaccharides from Eucalyptus Pre-hydrolysate
Received:November 05, 2021  
DOI：10.11980/j.issn.0254-508X.2022.04.005
Key Words:pre-hydrolysate  polysaccharides  separation and purification  antioxidant activity
Fund Project:国家自然科学基金青年科学基金项目（31700516）；天津市制浆造纸重点实验室开放基金（202005）。
Author NameAffiliationPostcode
LI Xuexiu China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
LIU Haitang* China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
AN Yongzhen China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
LIU Jing China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
CHEN Lin China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
JIN Xin China Light Industry Key Lab of Papermaking and Biorefinery， Tianjin Key Lab of Pulp and Paper， Tianjin University of Science & Technology， Tianjin， 300457 300457
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Abstract:The hemicellulose polysaccharides （PHLP） were extracted from the eucalyptus wood pre-hydrolysate （PHL） by alcohol precipitation， and were separated and purified by DEAE-650M ion exchange column. Meanwhile， the two purified components neutral polysaccharide PHLP-1 and acidic polysaccharide PHLP- 2 were analyzed. The results of monosaccharide components showed that both PHLP-1 and PHLP-2 were composed of arabinose， xylose， galactose， mannose and glucose， with a molar ratio of 0.09∶1∶0.13∶0.07∶0.35 and 0.1∶1∶0.03∶0.09∶0.08. The infrared spectroscopy showed that both components had characteristic absorption peaks of polysaccharides. The scanning electron microscopy analysis showed that the structures of PHLP-1 and PHLP-2 were disordered and messy. The results of antioxidant experiments showed that the two purified polysaccharides had certain antioxidant properties and there was a dose-effect relationship. The order of antioxidant ability was： PHLP-2>PHLP-1>PHLP.
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