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Study on the Improvement of Enzymatic Hydrolysis of Lignocellulose by Surfactant Under High Solid System
Received:July 07, 2020  
DOI：10.11980/j.issn.0254-508X.2020.12.006
Key Words:high solid system  enzymatic hydrolysis  sodium lignosulfonate  surfactant
Fund Project:天津市自然科学基金（18JCQNJC7570.0）；国家自然科学基金（21908167）;天津市高等学校大学生创新创业训练计划市级项目（201910057065）；国家级大学生创新创业训练计划项目（202010057003）。
Author NameAffiliationPostcode
GU Cheng Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
HUO Dan Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
HAN Xiao Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
CHEN Hui Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
YANG Qiulin Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
LI Lin Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
XUE Pengcheng Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
SI Chuanling Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
PEI Jicheng Tianjin Key Lab of Pulp & Paper， School of Light Industry Science and Engineering， Tianjin University of Science & Technology， Tianjin， 300457 300457
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Abstract:In this research， sodium lignosulfonate was used as an assistant to improve the enzymatic hydrolysis efficiency of Eucalyptus powders pretreated with metal chloride under high solid system. The results showed that the addition of sodium lignosulfonate promoted the enzymatic hydrolysis efficiency of the high solid system. When the amount of sodium lignosulfonate was 0.4%， the glucose yield from enzymatic hydrolysis could reach to 93.72%， which was 12.5 percent higher than that of blank sample. In order to further improve the efficiency of enzymatic hydrolysis， sodium lignosulfonate was compounded with CTAB and Tween 80. The results showed that the efficiency of enzymatic hydrolysis of high solid system was promoted synergistically. When m （CTAB）∶m （sodium lignosulfonate） was 1.5∶1， the cellulose conversion rate was 99.56%； when m （Tween 80）∶m （sodium lignosulfonate） was 1∶1， the cellulose conversion rate was 99.98%， so nearly total of the cellulose could be converted to glucose.
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