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| Preparation of Xylo-oligosaccharides from Eucalyptus Pre-hydrolysis Liquor with Xylanase Treatment |
| Received:May 03, 2019 |
| DOI：10.11980/j.issn.0254-508X.2019.08.001 |
| Key Words:eucalyptus pre-hydrolysis liquid xylo-oligosaccharides xylanase |
| Fund Project:国家重点研发计划资助项目 2017YFB0307900;国家自然科学基金资助项目 31670595 31770628国家重点研发计划资助项目（2017YFB0307900）；国家自然科学基金资助项目（31670595，31770628）；泰山学者工程。 |
| Author Name | Affiliation | Postcode | | 陈嘉川 | 齐鲁工业大学（山东省科学院）生物基材料与绿色造纸国家重点实验室/ 制浆造纸科学与技术教育部重点实验室,山东济南,250353 | 250353 | | 董吉冉 | 齐鲁工业大学（山东省科学院）生物基材料与绿色造纸国家重点实验室/ 制浆造纸科学与技术教育部重点实验室,山东济南,250353 | 250353 | | 杨桂花 | 齐鲁工业大学（山东省科学院）生物基材料与绿色造纸国家重点实验室/ 制浆造纸科学与技术教育部重点实验室,山东济南,250353 | 250353 | | 徐丰 | 齐鲁工业大学（山东省科学院）生物基材料与绿色造纸国家重点实验室/ 制浆造纸科学与技术教育部重点实验室,山东济南,250353 | 250353 | | 吉兴香 | 齐鲁工业大学（山东省科学院）生物基材料与绿色造纸国家重点实验室/ 制浆造纸科学与技术教育部重点实验室,山东济南,250353 | 250353 |
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| Abstract:In this paper, xylanase treatment was used to prepare xylo-oligosaccharides (XOS) from the secondary eucalyptus pre-hydrolysis liquor (PHL) which had been treated with Ca(OH)2 and activated carbon successively. The effects of xylanase dosage, treatment time, treatment temperature and pH value on XOSDP2~4 contents were investigated and the prepared XOS were characterized. The results showed that the optimal treatment conditions were xylanase dosage of 2 U/g (based on the weight of secondary eucalyptus PHL), treatment time of 3 h, treatment temperature of 55 ℃ and pH value of 5.5, The content of XOSDP2~4 of the third PHL was 12.22 g/L, increased by 67.2% compared to the secondary PHL, and the content of XOSDP2~4 of third PHL accounted for 56.1% of the total xylose in the eucalyptus PHL. Analysis of Fourier Transform infrared spectroscopy (FT-IR) and Thermo gravimetric (TGA) showed that XOS obtained with xylanase treatment process contained part of side-chains of uronic acid and showed a high thermal stability. |
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