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Study on the Removal of Lignin from Eucalyptus Pre-hydrolysis Liquid by Laccase Combined with Activated Carbon Treatment
  
DOI:10.11980/j.issn.0254-508X.2018.09.002
Key Words:Eucalyptus  pre-hydrolysis liquid  laccase  active carbon  small molecular lignin
Fund Project:国家重点研发计划项目(2017YFB0307900);国家自然科学基金(31470602,31500490,31670595,31770628);泰山学者工程。
Author NameAffiliation
DONG Ji-ran State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
CHEN Jia-chuan* State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
JI Xing-xiang State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
HE Ming State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
XU Feng State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
LIU Shan-shan State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
YANG Gui-hua* State Key Lab of Bio-based Materials and Green Papermaking Co-founded by Shandong and the Ministry of Science and Technology/Key Lab of Pulp & Paper Science and Technology of Education Ministry, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong Province, 250353 
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Abstract:In this work, laccase combined with activated carbon were used to remove small molecular lignin from secondary Eucalyptus pre-hydrolysis liquid which was treated with Ca(OH)2 and activated carbon successively. The effects of pH value, treatment temperature, laccase dosage and treatment time on lignin removal efficiency were analyzed. The main factors were analyzed and verified by single factor experiment and response surface method, and a mathematical model for the removal of small molecule lignin was established. The results showed that compared with secondary purified liquid without laccase treatment, the lignin removal rate increased by 12.8 percentage points after laccase treatment. And the 4 main factors affecting lignin removal rate from strong to weak were: pH treatment value of laccase >laccase dosage> treatment temperature>treatment time. Under the optimum treatment condition (pH=5.23, temperature 45.3℃, laccase dosage 5.68 U/g purified liquid and treatment time 190 min), the predicted maximum lignin removal rate in the liquid solution was 65.1%, and the actual measured value 65.1%. The lignin removal rate was 90.5% based on the Eucalyptus pre-hydrolysate.
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