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| Effect of Microbial Desorption from Porous Activated Carbon Materials for Papermaking Wastewater Treatment on DNA Extraction |
| Received:April 20, 2026 Revised:May 13, 2026 |
| DOI：10.11980/j.issn.0254-508X.2026.07.024 |
| Key Words:papermaking wastewater porous activated carbon composite mixture design microbial desorption DNA extraction |
| Fund Project:农业微生物学国家重点实验室开放课题（AMLKF201807）；食用野生植物保育与利用湖北省重点实验室开放基金（EWPL201709）。 |
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| Abstract:In papermaking wastewater treatment processes， when porous activated carbon composite materials were used as carriers to adsorb functional microbial， commercial DNA extraction kits faced technical challenges in inefficiently desorbing the microbial, making it difficult to accurately analyze the functional microbial communities. This study adopted a physicochemical desorption strategy, using the optimum mixture design feature in Design-Expert 13.0 software, a two-factor optimization design was conducted to determine desorption efforts of factor A (volume ratio of sodium pyrophosphate to rhamnolipid solutions) and factor B (mass ratio of zirconia beads with particle size of 0.1 and 1.0 mm). The results indicated that the established quadratic prediction model exhibited high significance (P<0.000 1), and there was a highly significant interaction between factor A and factor B (P<0.000 1). The optimum desorption conditions were determined that volume ratio of sodium pyrophosphate to rhamnolipid solutions of 1.00, and a mass ratio of zirconia beads with size of 0.1 and 1.0 mm of 1.00. Compared with five mainstream commercial DNA extraction kits, the DNA extracted using this optimized protocol exhibited higher concentration (365.33±8.33 ng/μL), higher purity (A260/A280=1.84±0.03， A260/A230=2.14±0.06), and higher integrity of large fragments, enabling efficient desorption of microorganisms. |
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