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| Optimization of Oxalic Acid Pretreatment of Wheat Straw for Hydrogen Production by Saccharification and Fermentation |
| Received:May 09, 2025 Revised:June 25, 2025 |
| DOI：10.11980/j.issn.0254-508X.2025.12.010 |
| Key Words:wheat straw oxalic acid response surface methodology mathematical model saccharification and fermentation |
| Author Name | Affiliation | Postcode | | DONG Cunjun* | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | ZENG Qiang | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | SUN Peng | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | YAN Li | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | ZHOU Yufeng | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | XIAO Chao | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | YANG Dewei | Wuhan Hongzhicai Packaging and Printing Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | HUANG Yuanjing | China Tobacco Hubei Industrial Co., Ltd., Wuhan, Hubei Province, 430040 | 430040 | | WANG Lei* | Hubei Provincial Key Lab of Green Materials for Light Industry, Hubei University of Technology, Wuhan, Hubei Province, 430068 | 430068 |
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| Abstract:This study used a Box-Behnken design and response surface methodology based on a three-variable central composite design to optimize the oxalic acid solution pretreatment process for wheat straw. A mathematical model was established to correlate the glucose conversion rate of wheat straw with pretreatment conditions, and its validity was validated. The results showed that reaction time and temperature during pretreatment were significant factors influencing the enzymatic hydrolysis efficiency of wheat straw. Through response surface analysis optimization, the optimal pretreatment conditions for glucose conversion were identified as follows: a molar ratio of water to oxalic acid of 3.8∶1.0, a reaction temperature of 110 ℃, and a holding time of 20 minutes. Under these conditions, the glucose conversion rate of cellulose reached 95.1%, similar to the model-predicted value (94.5%). Furthermore, the hydrogen production via simultaneous saccharification and fermentation (SSF) of pretreated wheat straw under these conditions achieved 131.5 mL/g, significantly higher than the biohydrogen yield from separate hydrolysis and fermentation (SHF) (105.2 mL/g). |
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