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| Study on the Effect of Hot Water + Green Liguor Pretreatment on the Enzymatic Hydrolysis of Bamboo Fibers to Prepare Fermentable Sugars |
| Received:November 25, 2024 Revised:June 23, 2025 |
| DOI：10.11980/j.issn.0254-508X.2025.11.008 |
| Key Words:pretreatment green liquor cellulase chemical additives fermentable sugars |
| Fund Project:湖南省科技创新计划项目（2024JK2080）。 |
| Author Name | Affiliation | Postcode | | WANG Jian* | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | WANG Xuchong* | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | LI Yong | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | SONG Huan | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | YANG Qing | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | DING Ping | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | ZHANG Ming | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 | | XU Yaomengli | Zhuzhou Times New Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412007
Zhuzhou Times Fiber Pioneer Material Technology Co., Ltd., Zhuzhou, Hu’nan Province, 412100 | 412100 |
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| Abstract:In this study， powdered single bamboo was pretreated with hot water and hot water + green liquor， combined with chemical additives such as bovine serum albumin (BSA) and Tween 80. The bamboo fibers were enzymatic hydrolyzed and saccharified by cellulase， and the effects of pretreatment conditions and chemical additives on the efficiency of enzymatic hydrolysis were investigated. The results showed that the two-step hot water + green liquor pretreatment had a significant effect on improving the enzymatic hydrolysis efficiency of bamboo fibers. When the total alkali content of green liquor was 14%， the total sugar conversion rate after enzymatic hydrolysis of hot water + green liquor pretreated bamboo fibers reached 85.51%. The addition of BSA and Tween 80 significantly increased the yield of fermentable sugars came from bamboo fibers， and the synergistic effect of the two additions could raise the total sugar conversion rate after enzymatic hydrolysis to 99.67%. Under the combined effects of hot water + green liquor pretreatment and chemical additives， the yield of fermentable sugars per 100 g of bamboo raw material could reach 65.88 g. |
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