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Study on Enzymatic Hydrolysis Improvement of Corn Stalk with Polyol-based Ternary DES Pretreatment
Received:September 25, 2024  
DOI：10.11980/j.issn.0254-508X.2024.12.003
Key Words:corn stalk  deep eutectic solvent  pretreatment  enzymatic hydrolysis  polyol
Fund Project:国家自然科学基金项目（22178028，U22A20422）；高等学校学科创新引智项目（B21022）。
Author NameAffiliationPostcode
XU Huimin Shandong Century Sunshine Paper Group Company Co.， Ltd.， Weifang， Shandong Province， 262400 262400
ZUO Yilan Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 100083
LI Haichao Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 100083
XU Feng* Beijing Key Lab of Lignocellulosic Chemistry， Beijing Forestry University， Beijing， 100083 100083
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Abstract:Effects of novel polyol-based deep eutectic solvent （DES） pretreatment on corn stalk components， surface morphology， polymerization degree （DP） of cellulose， and enzymatic efficiency were investigated. The results showed that polyol-based DES pretreatment could efficiently remove hemicellulose and acid insoluble lignin （AIL） from corn stalk and retain cellulose， but there was no positive correlation between the carbon chain length of polyol and pretreatment performance. Choline chloride/1，4-butanediol/4-hydroxy benzenesulfonic acid was the optimum polyol-based DES system， removing nearly 100% hemicellulose and 98% AIL， but saving 84% cellulose and DP=894. The surface of pretreated corn stalk was rough， porous， and no deposited lignin and pseudo-lignin particles. The corresponding enzymatic efficiency was as high as 94.80%， which was 5.9 times higher than that of raw corn stalk. In summary， the polyol-based DES pretreatment could greatly enhance the enzymatic performance by reducing cellulose DP， removing hemicellulose and AIL， and simultaneously inhibiting lignin redeposition and improving the surface roughness of corn stalk.
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