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| Hydrothermal Liquefaction Enzymolysis Coupling Technology for the Saccharification of Miscanthus Lutarioriparius Biomass |
| Received:April 14, 2024 |
| DOI：10.11980/j.issn.0254-508X.2024.11.008 |
| Key Words:miscanthus lutarioriparius liginocellulosic biomass hydrothermal liquefaction surfactant-assisted enzymatic hydrolysis biorefinery |
| Fund Project:国家自然科学基金（32000260）；中国博士后科学基金（2020M682566）；长沙市自然科学基金（kq2208068）。 |
| Author Name | Affiliation | Postcode | | YUAN Ran | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | LIANG Yinghong | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | FU Tongcheng | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | HU Shenglong | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | WANG Sheng | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | YI Zili | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 | | LI Meng* | College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Engineering Lab of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, Hu’nan Province, 410128
Hunan Branch, National Energy R&D Center for Non-food Biomass, Changsha, Hu’nan Province, 410128 | 410128 |
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| Abstract:To enhance the feedstock utilization rate of miscanthus lutarioriparius biomass， a hydrothermal liquefaction-surfactants enzymolysis coupling technology was designed to prepare xylooligosaccharides and other saccharides in this study. The reaction conditions were optimized using the response surface test and orthogonal experiments. During hydrothermal liquefaction stage， the highest saccharification efficiency could reach 59.38% under the conditions of 190 ℃， 10 min. Maximum yield of xylooligosaccharides， arabinose， and xylose were 15.53%，7.97%， and 52.58%， respectively. During the enzymatic hydrolysis stage， the highest efficiency of saccharification was 45.02% under the conditions of 30 FPU/g of cellulase and 8% of Tween-80. Treated by the two-stage reaction， the whole saccharification rate could reach 50.12% at maximum. |
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