Abstract:To enhance the feedstock utilization rate of miscanthus lutarioriparius biomass, a hydrothermal liquefaction-surfactants enzymolysis coupling technology was designed to prepare xylooligosaccharides and other saccharides in this study. The reaction conditions were optimized using the response surface test and orthogonal experiments. During hydrothermal liquefaction stage, the highest saccharification efficiency could reach 59.38% under the conditions of 190 ℃, 10 min. Maximum yield of xylooligosaccharides, arabinose, and xylose were 15.53%,7.97%, and 52.58%, respectively. During the enzymatic hydrolysis stage, the highest efficiency of saccharification was 45.02% under the conditions of 30 FPU/g of cellulase and 8% of Tween-80. Treated by the two-stage reaction, the whole saccharification rate could reach 50.12% at maximum. |