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Hydrothermal Liquefaction Enzymolysis Coupling Technology for the Saccharification of Miscanthus Lutarioriparius Biomass
Received:April 14, 2024  
DOI:10.11980/j.issn.0254-508X.2024.11.008
Key Words:miscanthus lutarioriparius  liginocellulosic biomass  hydrothermal liquefaction  surfactant-assisted enzymatic hydrolysis  biorefinery
Fund Project:国家自然科学基金(32000260);中国博士后科学基金(2020M682566);长沙市自然科学基金(kq2208068)。
Author NameAffiliationPostcode
YUAN Ran College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
LIANG Yinghong College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
FU Tongcheng College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
HU Shenglong College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
WANG Sheng College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
YI Zili College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
LI Meng* College of Bioscience and Biotechnology Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Engineering Lab of Miscanthus Ecological Applications Hunan Agricultural University Changsha Hu’nan Province 410128
Hunan Branch National Energy R&D Center for Non-food Biomass Changsha Hu’nan Province 410128 
410128
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Abstract:To enhance the feedstock utilization rate of miscanthus lutarioriparius biomass, a hydrothermal liquefaction-surfactants enzymolysis coupling technology was designed to prepare xylooligosaccharides and other saccharides in this study. The reaction conditions were optimized using the response surface test and orthogonal experiments. During hydrothermal liquefaction stage, the highest saccharification efficiency could reach 59.38% under the conditions of 190 ℃, 10 min. Maximum yield of xylooligosaccharides, arabinose, and xylose were 15.53%,7.97%, and 52.58%, respectively. During the enzymatic hydrolysis stage, the highest efficiency of saccharification was 45.02% under the conditions of 30 FPU/g of cellulase and 8% of Tween-80. Treated by the two-stage reaction, the whole saccharification rate could reach 50.12% at maximum.
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