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| Research on Determination of Guar Gum Content in Reconstituted Tobacco Sheets by Ion Chromatography |
| Received:November 29, 2023 |
| DOI：10.11980/j.issn.0254-508X.2024.05.018 |
| Key Words:ion chromatography reconstituted tobacco sheet guar gum |
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| Abstract:In this study， a method for the determination of guar gum content in reconstituted tobacco sheets by ion chromatography was established as follows： the guar gum in reconstituted tobacco sheets was extracted by aqueous soak and oscillation， the extract was evaporated to dryness， and then the guar gum was decomposed into galactose and mannose by the method of acid hydrolysis， and then the contents of galactose and mannose were determined by ion chromatography， and the guar gum content in the samples was obtained by the formulae for conversion. The results showed that the galactose and mannose had very good linear relationship in the concentration range of 0.1~10 mg/L with the correlation coefficients R2≥0.999 6， and the detection limits were 0.003 6 and 0.002 2 mg/L， respectively， and the recovery rates of guar gum were ≥95.8% with the RSD of 1.58 %. Therefore， the method had good repeatability， high accuracy and was suitable for the determination of guar gum content in reconstituted tobacco sheets. |
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