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Study on LiBr-assisted Ball Milling Pretreatment for Promoting the Efficiency of Enzymatic Hydrolysis of Corncob Residues to Glucose
Received:June 14, 2023  
DOI：10.11980/j.issn.0254-508X.2024.01.002
Key Words:corncob residue  pretreatment  lithium bromide  ball milling  enzymatic hydrolysis  glucose yield
Fund Project:国家自然科学基金（U22A20423）；山东能源研究院科研创新基金（SEI S202106）。
Author NameAffiliationPostcode
ZHANG Wenlu* Liaoning Key Lab of Lignocellulose Chemistry and BioMaterials， Liaoning Collaborative Innovation Center for Lignocellulosic Biorefinery， School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034
CAS Key Lab of Biofuels， Qingdao Institute of Bioenergy and Bioprocess Technology， Chinese Academy of Sciences， Qingdao， Shandong Province， 266101 		266101
LI Haiming Liaoning Key Lab of Lignocellulose Chemistry and BioMaterials， Liaoning Collaborative Innovation Center for Lignocellulosic Biorefinery， School of Light Industry and Chemical Engineering， Dalian Polytechnic University， Dalian， Liaoning Province， 116034 116034
YU Guang CAS Key Lab of Biofuels， Qingdao Institute of Bioenergy and Bioprocess Technology， Chinese Academy of Sciences， Qingdao， Shandong Province， 266101 266101
LI Bin* CAS Key Lab of Biofuels， Qingdao Institute of Bioenergy and Bioprocess Technology， Chinese Academy of Sciences， Qingdao， Shandong Province， 266101 266101
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Abstract:In this study， corncob residues （CCR） were used as raw materials to investigate the effects of LiBr-assisted ball milling pretreatment on enzymatic hydrolysis of CCR to glucose. Based on the monofactor analysis， it was concluded that the suitable conditions of LiBr-assisted ball milling pretreatment of CCR were as follows： ball milling time of 6.0 h， LiBr dosage of 50%， and solid content of CCR of 80%. Under the suitable pretreatment conditions， LiBr did not need water washing to separate， and efficient enzymatic hydrolysis of CCR was realized with lignin retention. At the cellulase dosage of 5 FPU/gCCR， the enzymatic glucose yield of pretreated CCR reached to 95%. The results of analysis and characterizations showed that LiBr-assisted ball milling could efficiently destroy the crystal structure of cellulose in CCR， reduce the crystallinity， make it have an amorphous structure， and increase the porosity of CCR， thus significantly promoting the enzymatic hydrolysis of CCR.
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