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Study on the Degradation Performance of Sodium Alginate-Agar-Montmorillonite Immobilized Bacteria on Reducing Phenol in Papermaking Wastewater |
Received:December 28, 2021 |
DOI:10.11980/j.issn.0254-508X.2022.03.005 |
Key Words:montmorillonite sodium alginate-agar phenol-degrading bacteria immobilization degradation kinetics |
Fund Project:国家自然科学基金“纳米Fe3O4/TiO2载体耦合转座子调控功能强化光合细菌处理预水解液的产氢研究”(项目批准号:52100193)。 |
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Abstract:In this paper, organic and inorganic coupling materials were used to embed immobilized phenol-degrading bacteria to construct immobilized microspheres, and the biodegradability of immobilized phenol-degrading bacteria on phenol in papermaking wastewater was studied. The results showed that the optimal conditions for the fixation of the bacteria Klebsiella pneumoniae ZS01 were: 1.0% sodium alginate, 1.5% montmorillonite, 1.0% agar, 25% bacterial inoculum, and the initial phenol concentration of 1000 mg/L. The phenol biodegradation rate of immobilized micropheres (16.67 mg/(L·h)) was significantly higher than that of free bacteria (13.89 mg/(L·h)). Further research found that under high phenol concentration, strong acid and strong alkali environment, the effect of immobilized micropheres to degrade phenol was significantly higher than that of free bacteria. In the study of phenol degradation kinetics, it was found that Haldane's model was in good agreement with the experimental data, indicating that high concentrations of phenol had a greater inhibitory effect on bacterial biodegradation. The immobilized micropheres were reused 32 times, and the phenol degradation efficiency was still as high as 99.5%, and stored at 4°C for 30 days, the phenol reduction efficiency of the immobilized micropheres remained above 99%. |
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