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Preparation of BC@Laccase Eliminate Material and Its Performance for 2-chlorophenol Removal in Water |
Received:January 28, 2021 |
DOI:10.11980/j.issn.0254-508X.2021.06.008 |
Key Words:bacterial cellulose immobilization laccase medium 2-chlorophenol |
Fund Project:浙江省重点研发计划项目(2020C04015);浙江省自然科学基金面上项目(LY19C160010);浙江省基础公益研究计划项目(LGF18C160002);制浆造纸工程国家重点实验室开放基金项目(201758);杭州市科技计划项目(20191203B28)。 |
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Abstract:In this study, glutaraldehyde was employed to fix laccase on bacterial cellulose (BC) substrate to prepare eco-friendly and reusable BC@Laccase eliminate materials by optimizing the crosslinking condition. FESEM, FT-IR, and TGA were used to characterize the obtained BC@Laccase product. The degradation performance of BC@Laccase on 2-chlorophenol in water was evaluated as well. The results showed that the optimal preparation process for the BC@Laccase was as follows:fixed time 4 h, fixed temperature 25℃, and pH value 3.5. The maximum catalytic degradation rate of 2-chlorophenol in water using BC@Laccase reached 76.9% under the catalytic conditions of erucic acid dosage of 4 mmol/L, degradation temperature of 30℃, pH value of 3, and degradation time of 5 h. Meanwhile, the catalytic degradation ability of BC@Laccase remained higher than 84.8% of its original capability after 7 cycles of use. |
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