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Preparation of BC@Laccase Eliminate Material and Its Performance for 2-chlorophenol Removal in Water
Received:January 28, 2021  
DOI：10.11980/j.issn.0254-508X.2021.06.008
Key Words:bacterial cellulose  immobilization  laccase  medium  2-chlorophenol
Fund Project:浙江省重点研发计划项目（2020C04015）；浙江省自然科学基金面上项目（LY19C160010）；浙江省基础公益研究计划项目（LGF18C160002）；制浆造纸工程国家重点实验室开放基金项目（201758）；杭州市科技计划项目（20191203B28）。
Author NameAffiliationPostcode
CHEN Jie Company Limited of Ce'an Energy Technology， Shanghai， 201822 201822
YANG Yun Company Limited of Ce'an Energy Technology， Shanghai， 201822 201822
HU Xueyi College of Materials Science and Engineering， Zhejiang Sci-Tech University， Hangzhou， Zhejiang Province， 310018 310018
ZHANG Yong College of Materials Science and Engineering， Zhejiang Sci-Tech University， Hangzhou， Zhejiang Province， 310018 310018
GONG Yu Nuclear and Radiation Safety Center， Ministry of Ecology and Environment of the People’s Republic of China， Beijing， 100082 100082
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Abstract:In this study， glutaraldehyde was employed to fix laccase on bacterial cellulose （BC） substrate to prepare eco-friendly and reusable BC@Laccase eliminate materials by optimizing the crosslinking condition. FESEM， FT-IR， and TGA were used to characterize the obtained BC@Laccase product. The degradation performance of BC@Laccase on 2-chlorophenol in water was evaluated as well. The results showed that the optimal preparation process for the BC@Laccase was as follows：fixed time 4 h， fixed temperature 25℃， and pH value 3.5. The maximum catalytic degradation rate of 2-chlorophenol in water using BC@Laccase reached 76.9% under the catalytic conditions of erucic acid dosage of 4 mmol/L， degradation temperature of 30℃， pH value of 3， and degradation time of 5 h. Meanwhile， the catalytic degradation ability of BC@Laccase remained higher than 84.8% of its original capability after 7 cycles of use.
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