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| Effect of Inhibitors Produced by Pretreatment Lignocellulosic Materials on Cellulase Enzymatic Efficiency |
| Received:March 04, 2020 |
| DOI：10.11980/j.issn.0254-508X.2020.06.004 |
| Key Words:bioenergy inhibitor cellulose enzymatic |
| Fund Project:国家重点研发计划课题（编号：2019YFB1503801）；湖南省科技重大专项项目（编号：2017NK1010）；湖南省重点研发计划项目（编号：2017WK2022）。 |
| Author Name | Affiliation | Postcode | | LI Ming | School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hu’nan Province, 410004
Ministry of Forestry Bioethanol Research Center, Changsha, Hu’nan Province, 410004
Hu’nan Engineering Research Center for Woody Biomass Conversion, Changsha, Hu’nan Province, 410004
Hu’nan International Joint Lab of Woody Biomass Conversion,Changsha,Hu’nan Province,410004）
（*E-mail：pzhan 1982@163.com
chenjnx@163.com | 410004 | | ZHAN Peng | School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hu’nan Province, 410004
Ministry of Forestry Bioethanol Research Center, Changsha, Hu’nan Province, 410004
Hu’nan Engineering Research Center for Woody Biomass Conversion, Changsha, Hu’nan Province, 410004
Hu’nan International Joint Lab of Woody Biomass Conversion,Changsha,Hu’nan Province,410004）
（*E-mail：pzhan 1982@163.com
chenjnx@163.com | 410004 | | CHEN Jienan | School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hu’nan Province, 410004
Ministry of Forestry Bioethanol Research Center, Changsha, Hu’nan Province, 410004
Hu’nan Engineering Research Center for Woody Biomass Conversion, Changsha, Hu’nan Province, 410004
Hu’nan International Joint Lab of Woody Biomass Conversion,Changsha,Hu’nan Province,410004）
（*E-mail：pzhan 1982@163.com
chenjnx@163.com | 410004 | | WANG Yanan | School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hu’nan Province, 410004
Ministry of Forestry Bioethanol Research Center, Changsha, Hu’nan Province, 410004
Hu’nan Engineering Research Center for Woody Biomass Conversion, Changsha, Hu’nan Province, 410004
Hu’nan International Joint Lab of Woody Biomass Conversion,Changsha,Hu’nan Province,410004）
（*E-mail：pzhan 1982@163.com
chenjnx@163.com | 410004 | | ZHOU Yongcai | School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hu’nan Province, 410004
Ministry of Forestry Bioethanol Research Center, Changsha, Hu’nan Province, 410004
Hu’nan Engineering Research Center for Woody Biomass Conversion, Changsha, Hu’nan Province, 410004
Hu’nan International Joint Lab of Woody Biomass Conversion,Changsha,Hu’nan Province,410004）
（*E-mail：pzhan 1982@163.com
chenjnx@163.com | 410004 |
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| Abstract:In the study of ethanol production from lignocellulosic materials, the raw materials are prone to produce inhibitors such as furfural, 5-hydroxymethylfurfural, hydroquinone, and resorcinol during the pretreatment process of dilute phosphoric acid impregnation and steam explosion. These inhibitors will affect the cellulase enzymatic hydrolysis efficiency resulting to a reduction of reducing sugar concentration and ethanol production. Through single-factor experiments, the optimal enzymatic conditions for cellulase enzymatic hydrolysis were determined as enzymatic hydrolysis temperature of 50℃, enzymatic solution dosage of 30 U/mL, solid-liquid ratio of the enzymatic hydrolysis system of 1∶11, and pH value of enzymatic hydrolysis system of 4.8. The contents of above four kinds of inhibitors produced during the pretreatment process were determined, which were 0.031 g/L for furfural, 0.029 g/L for 5-hydroxymethylfurfural, 1.51 g/L for hydroquinone, and Hydroquinone 1.242 g/L. The effect of the above inhibitors alone or in combination on the enzymatic hydrolysis efficiency of cellulose was expolred. The results showed that the phenolic inhibitors produced during the pretreatment process were more than the aldehydes, and the influence of the phenolic inhibitors on the enzymatic hydrolysis was higher than the aldehyde inhibitors during the enzymatic hydrolysis. The synergistic effect of multiple inhibitors on the degradation rate of cellulose was higher than the effect of a single inhibitor. The influence intensity of the inhibitors on enzymolysis in the enzymatic hydrolysis solution from strong to weak was in order of hydroquinone, resorcinol, and 5-hydroxymethylfurfural , and furfural. |
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