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Effect of Auto-hydrolysis Pretreatment on Xylan Distribution in the Cell Walls of Poplar Chips
  
DOI：10.11980/j.issn.0254-508X.2016.07.001
Key Words:auto-hydrolysis pretreatment  xylan in poplar chips  immunofluorescence  confocal laser scanning microscopy
Fund Project:本课题得到国家自然科学基金项目(31270630)的资助。
Author NameAffiliation
耿星月 天津科技大学天津市制浆造纸重点实验室,天津,300457 
刘　苇 天津科技大学天津市制浆造纸重点实验室,天津,300457 
侯庆喜* 天津科技大学天津市制浆造纸重点实验室,天津,300457 
陈　威 天津科技大学天津市制浆造纸重点实验室,天津,300457 
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Abstract:The change of xylan distribution in the cell wall of poplar chips caused by auto-hydrolysis pretreatment was studied by using immunofluorescence combined with confocal laser scanning microscopy (CLSM). A gradual loss of xylan antibody signals in the center of the poplar cell wall was observed but only a slight loss at the edge or even a slight increase at some places of the cell walls were found as the intensity factor of auto-hydrolysis pretreatment increased. HPLC analysis was also conducted to measure the overall change of xylan content. As the intensity factor increased to 3.72, xylan content decreased from 14.64% to 7.33%. The change of the xylan signal intensity from spectra of the CLSM had the same trend as that of the xylan content determined by using the HPLC analysis.
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