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| Biosynthesis of Chitosan Using Xylose from Hemicellulose Hydrolysate |
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| DOI：10.11980/j.issn.0254-508X.2015.05.007 |
| Key Words:chitosan hemicellulose pulping effluent detoxification Actinomucor elegans |
| Author Name | Affiliation | | 林广修 | 天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津,300457 | | 赵华 | 天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津,300457 | | 刘盼 | 天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津,300457 | | 车利伟 | 天津科技大学生物工程学院,工业发酵微生物教育部重点实验室,天津,300457 |
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| Abstract:The detoxification technology and culture conditions of biosynthesis of chitosan using xylose from hemicelluloses hydrolysate such as pulping waste liquor were investigated. The detoxification technology was as follows: pH was adjusted to 10 with Ca(OH)2 at first then adjusted back to 7 with 10% H2SO4 after suction filtration, then activated carbon was added in a ratio of 1∶20(W∶V) after the second suction filtration, finally it was put on a rocking table at the tempreture of 50℃ and the rotating speed of 120 r/min for 2 hours, the supernatant after the final suction filtration was collected for cultivation. The culture conditions included that using 6% of corn milk as nitrogen source and properly diluted detoxicated pulping waste liquor (which contains 3% of xylose) as carbon source. Actinomucor elegans(TCCC450005) was cultured in this medium at 30℃ for 60 hours. The mycelia concentration was 15.20 g/L and the chitasan yield was 2.02 g/L. |
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