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Effects of Sample Preparation on the Detection of Colonies Number in Sanitary Tissue
  
DOI：10.11980/j.issn.0254-508X.2013.04.008
Key Words:sanitary tissue  plate counting  total colony  sample preparation
Fund Project:本课题得到国家自然科学基金（21076091）和国家质检总局科技计划（质量技术监督）项目（2011QK303)资助。
Author NameAffiliation
田迎新1 1.华南理工大学制浆造纸工程国家重点实验室，广州广东，510641 
胡会超1 1.华南理工大学制浆造纸工程国家重点实验室，广州广东，510641 
柴欣生1,2,* 1.华南理工大学制浆造纸工程国家重点实验室，广州广东，510641
2.国家纸制品质量监督检验中心,广东东莞，523080 
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Abstract:This paper investigated the effects of the pulp consistency, the types of culture medium, the way of disintegration, and the addition amount of surfactant in sample preparation on the detection of colonies number in sanitary tissue by plate counting method. The result showed that the optimum conditions of sample preparation are as follows: 1% pulp consistency, using TGEA culture medium, using disintegrator jar for integrating sample, and in the presence of 10 μL/L tween-80, from which the sensitivity and precision of colonies number detection for sanitary tissue can be improved.
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