| Abstract:Rubbings are important documents in China. They serve as important carriers of the nation"s cultural history. Therefore, conservation research is of great importance for the long-term preservation of rubbings. In view of the fact that there is a large amount of mould growth in the rubbings of the Zhang Qian Memorial Hall in Chenggu County, in this study the moulds were isolated and purified using PDA culture medium. Then, for the scientific identification of the moulds, a combination of micromorphological observation and 18S rDNA-ITS sequence analysis was used. The results showed that the predominant mould species found in this batch of rubbings were Aspergillus niger, Trichoderma, Penicillium and Aspergillus flavus. Different concentrations of nano-zinc oxide were then investigated for their antifungal performance. The macroscopic observation and the microscopic observation based on the scanning electron microscope showed that 2.0% nano zinc oxide exhibited effective anti-fungal properties on the paper samples. These results provide a valuable insight for the conservation efforts in relation to this particular batch of rubbings. |