| Abstract:In order to achieve efficient enrichment and detection of trace fentanyl-like compounds in water environment, this paper prepared a cellulose paper-based extraction material modified by diphenylmethane diisocyanate (MDI) through chemical grafting method. Combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis technology, a highly efficient extraction and quantitative detection method for 7 fentanyl-like compounds in water was constructed. Scanning electron microscopy (SEM), infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA) indicated that MDI successfully reacted with the hydroxyl groups on the cellulose surface and formed an amine carbamate (polyurethane) structure. This extraction material adsorbs fentanyl-like compounds through hydrophobicity and π-π interactions. The optimal extraction conditions were determined as follows: the sample pH was 9.0, the elution solvent was acetone, and the adsorption equilibrium time was 40 min. All fentanyl-like compounds exhibited good linearity in the HPLC-MS/MS analysis within the concentration range of 1–100 μg·L?¹, with correlation coefficients R² > 0.99. At three spiked concentrations, the average spiked recovery of the seven fentanyl-like compounds in actual water samples ranged from 53.0% to 106%, with detection limits (LOD) between 0.02 and 0.07 μg·L?¹. These results indicate that the MDI-cellulose paper-based extraction material exhibits excellent adsorption and enrichment performance for fentanyl derivatives. |