| Abstract:The effects of novel polyol-based deep eutectic solvent (DES) pretreatment on corn stover components, surface morphology, degree of cellulose polymerization (DP) and enzymatic efficiency were investigated. The results showed that polyol-based DES could efficiently remove hemicellulose and acid soluble lignin (AIL) from corn stalk and retain cellulose, but there was no positive correlation between the polyol carbon chain and pretreatment performance. Choline chloride/1, 4-butanediol/4-hydroxy benzenesulfonic acid was the optimal system, removing nearly 100% hemicellulose and 98% AIL, but saving 84% cellulose with 894 of the DP value. The surface of corn stalk was rough and porous and no deposited lignin and pseudo-lignin particles. The enzyme digestion efficiency was as high as 94.80%, which was 5.9 times higher than that of the raw material. The polyol-based DES pretreatment greatly enhanced the enzymatic performance by reducing cellulose polymerization, removing hemicellulose and AIL, and simultaneously inhibiting lignin redeposition and improving the surface roughness of corn stalk. |