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生物促生剂对废纸造纸活性污泥胞外聚合物的影响
The Effects of Bio-stimulant on OCC Papermaking Activated Sludge Extracellular Polymeric Substances
收稿日期：  
DOI：10.11980/j.issn.0254-508X.2017.01.005
关键词:  生物促生剂  胞外聚合物  松散结合的胞外聚合物  紧密结合的胞外聚合物
Key Words:bio-stimulant(BS)  extracellular polymeric substances(EPS)  loosely bound extracellular polymeric substances(LB-EPS)  tightly bound extracellular polymeric substances(TB-EPS)
基金项目:制浆造纸工程国家重点实验室开放基金(201604)。
作者单位
张安龙1 1.陕西科技大学环境科学与工程学院,陕西西安，710021 
周丹妮1,* 1.陕西科技大学环境科学与工程学院,陕西西安，710021 
杜　飞2 2.陕西科技大学轻工科学与工程学院,陕西西安，710021 
郗文君1 1.陕西科技大学环境科学与工程学院,陕西西安，710021 
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摘要:在造纸废水处理过程中加入生物促生剂是为了促进污染物降解,最终达到去除废水中污染物的目的。本研究通过对废纸造纸废水活性污泥胞外聚合物(EPS)、松散结合的胞外聚合物(LB-EPS)、紧密结合的胞外聚合物(TB-EPS)的研究,来证明其中某种成分可以作为指示生物促生剂用量的指示剂,以及与污泥沉降性能的相关性。实验采用３种不同CODCr值的造纸废水,并取３组不同用量的生物促生剂分别进行实验。通过采用硫酸法、离心和声波降解法分别提取污泥中EPS、LB-EPS、TB-EPS,用分光光度法测定其中蛋白质、多糖、腐殖质的量,以此确定生物促生剂最佳用量；并采用生物促生剂处理废水的正交实验进行验证。结果表明,不同CODCr的废水,存在不同的最佳生物促生剂用量；真正影响生物促生剂用量和污泥沉降性能控制参数的是TB-EPS中的蛋白质的量和LB-EPS中的蛋白质的量。
Abstract:In the process of OCC papermaking wastewater treatment, the bio-stimulant was added to promote the degradation of pollutants, and finally to remove the pollutants from wastewater. Based on the study of extracellular polymers(EPS), loosely bound extracellular polymeric substances(LB-EPS), and tightly bound extracellular polymeric substances(TB-EPS) of activated sludge of OCC papermaking wastewater, to prove some components could be used as indicator of bio-stimulant dosage which was related to sludge settleability. Experiment using three different COD load of papermaking wastewater, and three groups of different dosages of bio-stimulant were used to carry out the experiment. In order to determine the optimum dosage of bio-stimulant the EPS, LB-EPS, TB-EPS were extract through sulfuric acid method, centrifugal method and ultrasonic wave decomposition method and measured the amount of proteins, polysaccharides and humus of sludge using spectrophotometry. Finally orthogonal experiment of bio-stimulant wastewater treatment was conduct for verification. The results showed that under different inlet COD load, there were different optimal dosage of bio-stimulant. The conclusion was that protein contents in LB-EPS and TB-EPS were the real impact factors on bio-stimulants dosage and sludge settleability control parameters．
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